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primary antibody α-cdk5 #sc-6247  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology primary antibody α-cdk5 #sc-6247
    Primary Antibody α Cdk5 #Sc 6247, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdk5+primary+antibody/anti+cyclin+d1/pmc09909437-271-2-10
    Average 90 stars, based on 1 article reviews
    primary antibody α-cdk5 #sc-6247 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Lysis:

    Article Title: Tumor Expression of Cyclin-Dependent Kinase 5 (Cdk5) Is a Prognostic Biomarker and Predicts Outcome of Oxaliplatin-Treated Metastatic Colorectal Cancer Patients
    Article Snippet: .. Cells were homogenized in lysis buffer and the supernatant was incubated with a Cdk5 primary antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA; (J3): sc-6247) overnight at 4 °C. .. The mix was incubated 2 hours with protein G-coupled beads (Millipore, St. Louis, MO, USA) and the beads were washed and boiled for 5 minutes in a loading buffer to detach the protein complex.

    Incubation:

    Article Title: Tumor Expression of Cyclin-Dependent Kinase 5 (Cdk5) Is a Prognostic Biomarker and Predicts Outcome of Oxaliplatin-Treated Metastatic Colorectal Cancer Patients
    Article Snippet: .. Cells were homogenized in lysis buffer and the supernatant was incubated with a Cdk5 primary antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA; (J3): sc-6247) overnight at 4 °C. .. The mix was incubated 2 hours with protein G-coupled beads (Millipore, St. Louis, MO, USA) and the beads were washed and boiled for 5 minutes in a loading buffer to detach the protein complex.



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    Image Search Results


    Figure 1: Both CDK5 inhibition and depletion affect the phosphorylation level of Ser126-Pit-1. (a) Bioinformatics analysis reveals that Ser126 Pit-1 was the only typical CDK5 potential phosphorylation site. (b) GH3 pituitary cells were transfected with WT-Pit-1 or Pit-1-126A. Western blotting shows that the anti-pS126-Pit-1 antibody can specifically recognize the phosphorylation of Ser126-Pit-1. (c). Western blot (left) and densitometry analysis (right) showed that S126-Pit-1 phosphorylation was reduced by roscovitine treatment at the indicated concentra- tions. The protein levels of Pit-1 was not clearly different. β-Actin served as a loading control. (d) Western blot showed that Ser126-Pit-1 phosphorylation was reduced by CDK5 siRNA treatment at the indicated concentrations. β-Actin served as a loading control. **, P < 0.01; ***, P < 0.001. The bar represents the mean ± SD.

    Journal: Open Chemistry

    Article Title: Phosphorylation of Pit-1 by cyclin-dependent kinase 5 at serine 126 is associated with cell proliferation and poor prognosis in prolactinomas

    doi: 10.1515/chem-2021-0070

    Figure Lengend Snippet: Figure 1: Both CDK5 inhibition and depletion affect the phosphorylation level of Ser126-Pit-1. (a) Bioinformatics analysis reveals that Ser126 Pit-1 was the only typical CDK5 potential phosphorylation site. (b) GH3 pituitary cells were transfected with WT-Pit-1 or Pit-1-126A. Western blotting shows that the anti-pS126-Pit-1 antibody can specifically recognize the phosphorylation of Ser126-Pit-1. (c). Western blot (left) and densitometry analysis (right) showed that S126-Pit-1 phosphorylation was reduced by roscovitine treatment at the indicated concentra- tions. The protein levels of Pit-1 was not clearly different. β-Actin served as a loading control. (d) Western blot showed that Ser126-Pit-1 phosphorylation was reduced by CDK5 siRNA treatment at the indicated concentrations. β-Actin served as a loading control. **, P < 0.01; ***, P < 0.001. The bar represents the mean ± SD.

    Article Snippet: Primary antibodies anti-CDK5 (ab40773, 1/200) were obtained from Abcam (Cambridge, MA, USA).

    Techniques: Inhibition, Phospho-proteomics, Transfection, Western Blot, Control

    Figure 2: pSer126-Pit-1 promotes GH3 cell proliferation and suppresses apoptosis. (a) Western blot showed pSer126-Pit-1 in GH3 cell with WT-Pit-1 or Pit-1-126A. (b) The colony formation test shows that the percentage of colonies formed was reduced in the Pit-1-126A groups compared with that of the Pit-1 groups. (c) The apoptosis rates of the Pit-1-126A groups were significantly higher than those of the Pit-1 groups. (d) Higher cell viability was observed in the Pit-1 groups. (e) The effect of CDK5 could be reversed by Pit-1 siRNA. **, P < 0.01; ***, P < 0.001. The bar represents the mean ± SD.

    Journal: Open Chemistry

    Article Title: Phosphorylation of Pit-1 by cyclin-dependent kinase 5 at serine 126 is associated with cell proliferation and poor prognosis in prolactinomas

    doi: 10.1515/chem-2021-0070

    Figure Lengend Snippet: Figure 2: pSer126-Pit-1 promotes GH3 cell proliferation and suppresses apoptosis. (a) Western blot showed pSer126-Pit-1 in GH3 cell with WT-Pit-1 or Pit-1-126A. (b) The colony formation test shows that the percentage of colonies formed was reduced in the Pit-1-126A groups compared with that of the Pit-1 groups. (c) The apoptosis rates of the Pit-1-126A groups were significantly higher than those of the Pit-1 groups. (d) Higher cell viability was observed in the Pit-1 groups. (e) The effect of CDK5 could be reversed by Pit-1 siRNA. **, P < 0.01; ***, P < 0.001. The bar represents the mean ± SD.

    Article Snippet: Primary antibodies anti-CDK5 (ab40773, 1/200) were obtained from Abcam (Cambridge, MA, USA).

    Techniques: Western Blot